Visual Cortex Neuron

Visual-cortex-neuron

This is a fluorescently tagged primary visual cortex neuron from a light microscope capture of a brain slice preparation.  These data were focus stacked revealing a tremendous amount of detail including the spines.  Focus stacking is common method in confocal microscopy, but many folks do not realize that you can also do it with traditional light microscopy of just about anything using ImageJ or Adobe Photoshop.  See here for an example and discussion of focus stacking.

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Silicone Oil In Anterior Chamber

SILICONE OIL

This image shows a slit lamp retro-illumination photograph through dilated pupil revealing silicone oil in anterior chamber following retinal re-attachment surgery.  Silicone oil is a commonly used approach for retinal tamponade during surgery in the vitreous or for retinal reattachment surgery.  While the silicone does assist with successful reattachment, often it causes optical complications resulting in followup surgeries to remove the silicone oil typically 2-8 months later.  Gas bubbles with SF6 or C3F8 are also used, with the advantage that there is no myopic shift post operation and no followup surgery is required.

This image was taken by Paula Morris of the Moran Eye Center using a  Zeiss photo slitlamp and a Nikon D-1S camera at and 24x magnification.  Notably, this image won Honorable mention in the Photo Slit Lamp Biomigraography Division in 2007.

Happy Holidays 2012 And A Happy New Year In 2013

First Light Connectomics

We at Webvision would like to wish you all a very happy holiday season and a happy New Year in 2013.  Continuing on the theme from last years holiday season wishes, we have for you a colorful holiday image titled “Connectomics, First Light” from Robert E. Marc utilizing data generated by Scott Lauritzen, Crystal Sigulinsky and John Vo Hoang.

What we are seeing is a necklace of coupled retinal bipolar cells linked by sparse suboptical gap junctions, fundamentally new circuitry for retinal processing.  There is much more to this story, but we’ll wait until the publication.  For now, consider these data generated from the Connectomics project in the Marclab at the Moran Eye Center to be a colorful holiday image that represents our best wishes to you.

Nikon Small World 2012 Finalist Michael Bridge

The University of Utah‘s own Michael Bridge from the HSC Core Research Facilities, Cell Imaging Lab at the University of Utah is a finalist in Nikon’s Small World Competition for 2012 and represents the vision community in this competition for us with his 7th place photography entry of a Drosophila melanogaster eye organ (third-instar larvae).

Congratulations to all the winners, but from us here at Webvision, another special congratulations to Michael!

Subject: Eye organ of a Drosophila melanogaster (third-instar larvae)
Technique: Confocal
Magnification: 60x

Nikon Small World 2012 Finalist Ryan Williamson

W. Ryan Williamson from the Howard Hughes Medical Institute (HHMI), Ashburn, Virginia is a finalist in Nikon’s Small World Competition for 2012 and represents the vision community in this competition for us with his 4th place photography entry of a Drosophila melanogaster visual system.

Congratulations to all the winners, but from us here at Webvision, special congratulations to Ryan!

Subject: Drosophila melanogaster visual system halfway through pupal development, showing retina (gold), photoreceptor axons (blue), and brain (green)
Technique: Confocal
Magnification: 1,500x

Astrocytes and Vasculature

This laser confocal image shows a GFP transgenic mouse retina under the control of the GFAP promoter stained with anti-Collagen IV (blue), anti-GFAP (red) and anti-GFP (green).  These labels not only show the spatial relationship of individual astrocytes to one another, but also the vasculature.   Image provided by Gabriel Luna out of the Steve Fisher and Geoff Lewis’s retinal cell biology group at UC Santa Barbara Neuroscience Research Institute.

Complicated Retinal Detatchment

This set of images (click the image above to enlarge) are optical coherence tomography (OCT) images of retained perfluoro-octane (PFO)  droplets after repair of a complicated retinal detachment.   PFO is a good surgical tool to help reattach the retina as they force sub retinal fluid out of any retinal tears and helps adhere the retina to the back of the globe.  PFO also helps localize the margins of retinal detachments, and reduces the height of the retinal detachment which assists in surgical laser photocoagulation.   Per Dr. Bernstein, “These subretinal droplets of PFO can remain in place indefinitely and cause minimal visual symptoms as long as they are not under or near the fovea.”

Images graciously provided to Webvision by Cyrie Fry of the Moran Eye Center.

Retinal Half Mosaic

This beautiful image is another by Gabriel Luna out of Steve Fisher and Geoff Lewis’s retinal cell biology group at UC Santa Barbara Neuroscience Research Institute that shows a small mosaic of the outer plexiform layer in mouse retina stained with anti-Calbindin D (green; horizontal cells) PNA (red; cone terminals) and GFP for bipolar cells (blue).  I love the regular order that the retina shows, yet its beauty and regularity belie the complexity that is present.