We have another beautiful image (larger size image here) from Gabe Luna in Steve Fisher’s and Geoff Lewis‘ group. This image representing the evolution of disease post retinal detachment, earned Honorable Mention in the 2013 Olympus Bioscapes International Digital Imaging Competition.
The image is a 60x image using laser scanning confocal image of the murine retinal nerve fiber layer after 2 weeks after retinal detachment. The ganglion cell axons (red), astrocytes (green), and blood vessels (blue) are labeled using SMI-32, GFAP, and Collagen IV antibodies respectively.
This laser confocal image shows a GFP transgenic mouse retina under the control of the GFAP promoter stained with anti-Collagen IV (blue), anti-GFAP (red) and anti-GFP (green). These labels not only show the spatial relationship of individual astrocytes to one another, but also the vasculature. Image provided by Gabriel Luna out of the Steve Fisher and Geoff Lewis’s retinal cell biology group at UC Santa Barbara Neuroscience Research Institute.
Another amazing image sent to us by Gabriel Luna out of the Steve Fisher and Geoff Lewis’s retinal cell biology group at UC Santa Barbara Neuroscience Research Institute. This image is of the optic nerve head of a normal mouse retina displaying the “glial tubes” formed by the astrocytic network (anti-GFAP; red). Anti-GFP (green) and anti-Collagen IV (blue) which were used to determine numbers of astrocytes and relative locations in relation to blood vessels.