We at Webvision would like to wish you the very best this holiday season. As in past years, we like to post an image from retinal science that is somehow evocative of the Holiday Season and this year, Gabe Luna from the Steve Fisher / Geoff Lewis laboratory delivers a stunning image of astrocytes in a retinal flat mount, but with a twist… We think you’ll be seeing more of Gabe’s beautiful imagery, but for now, here is his description of how he made this image:
“I used a GFAP-GFP mouse to identify all the astrocytes in the retina and manually (at the time it was manual) annotate their coordinates, then we used a probabilistic random-walk algorithm to go to each “cell center” and perform a segmentation result of that one astrocyte. Once all the 5,000 or so cells are segmented as a greyscale image of the individual cell, then they are assigned various hues that are spectrally distinct and the montage is re-assembled into one large image. The image there is a grossly down-sized image of the original. The original was a seamless mosaic of 412 individual z-stacks of about 15 planes at 1 micron intervals, using a 40x oil immersion lens.”
Happy Holidays from Webvision. We wish you a happy, healthy and productive holiday season and New Year.
Gabe Luna from Steve Fisher and Geoff Lewis’s retinal cell biology group at UC Santa Barbara Neuroscience Research Institute provided this festive laser confocal image of a wholemount from a normal retina with dye-filled retinal astrocytes using Lucifer Yellow (green) and Alexa Fluor 568 (red) which were used to examine the spatial organization between individual astrocytes.
We have another beautiful image (larger size image here) from Gabe Luna in Steve Fisher’s and Geoff Lewis‘ group. This image representing the evolution of disease post retinal detachment, earned Honorable Mention in the 2013 Olympus Bioscapes International Digital Imaging Competition.
The image is a 60x image using laser scanning confocal image of the murine retinal nerve fiber layer after 2 weeks after retinal detachment. The ganglion cell axons (red), astrocytes (green), and blood vessels (blue) are labeled using SMI-32, GFAP, and Collagen IV antibodies respectively.
This image from Scott McLeod from Jerry Lutty’s lab, is a wholemount human retina preparation triple labeled with fluorescent antibodies that stain blood vessels (blue), astrocytes (red) and microglia (green). The specimen was imaged on a Zeiss 710 Confocal Microscope and is merged from 46 optical Z sections.
It originally appeared on Flickr here. You can see more of Scott’s work on his Flickrstream.