We at Webvision would like to wish you the very best this holiday season. As in past years, we like to post an image from retinal science that is somehow evocative of the Holiday Season and this year, Gabe Luna from the Steve Fisher / Geoff Lewis laboratory delivers a stunning image of astrocytes in a retinal flat mount, but with a twist… We think you’ll be seeing more of Gabe’s beautiful imagery, but for now, here is his description of how he made this image:
“I used a GFAP-GFP mouse to identify all the astrocytes in the retina and manually (at the time it was manual) annotate their coordinates, then we used a probabilistic random-walk algorithm to go to each “cell center” and perform a segmentation result of that one astrocyte. Once all the 5,000 or so cells are segmented as a greyscale image of the individual cell, then they are assigned various hues that are spectrally distinct and the montage is re-assembled into one large image. The image there is a grossly down-sized image of the original. The original was a seamless mosaic of 412 individual z-stacks of about 15 planes at 1 micron intervals, using a 40x oil immersion lens.”
We have another beautiful image (larger size image here) from Gabe Luna in Steve Fisher’s and Geoff Lewis‘ group. This image representing the evolution of disease post retinal detachment, earned Honorable Mention in the 2013 Olympus Bioscapes International Digital Imaging Competition.
The image is a 60x image using laser scanning confocal image of the murine retinal nerve fiber layer after 2 weeks after retinal detachment. The ganglion cell axons (red), astrocytes (green), and blood vessels (blue) are labeled using SMI-32, GFAP, and Collagen IV antibodies respectively.
This laser confocal image shows a GFP transgenic mouse retina under the control of the GFAP promoter stained with anti-Collagen IV (blue), anti-GFAP (red) and anti-GFP (green). These labels not only show the spatial relationship of individual astrocytes to one another, but also the vasculature. Image provided by Gabriel Luna out of the Steve Fisher and Geoff Lewis’s retinal cell biology group at UC Santa Barbara Neuroscience Research Institute.
This poster was presented today at the Association for Research in Vision and Opthalmology (ARVO) meetings in Ft. Lauderdale, Florida by W. Drew Ferrell, Lloyd Williams, Carl B. Watt, James R. Anderson, Robert E. Marc and Bryan William Jones. Full size (almost) poster can be seen here.
Another amazing image sent to us by Gabriel Luna out of the Steve Fisher and Geoff Lewis’s retinal cell biology group at UC Santa Barbara Neuroscience Research Institute. This image is of the optic nerve head of a normal mouse retina displaying the “glial tubes” formed by the astrocytic network (anti-GFAP; red). Anti-GFP (green) and anti-Collagen IV (blue) which were used to determine numbers of astrocytes and relative locations in relation to blood vessels.
This paper by Vazquez-Chona FR, Swan A, Ferrell WD, Jiang L, Baehr W, Chien WM, Fero M, Marc RE and Levine EM addresses a long standing issue in the field of neuroscience: is the reactive phenotype of glial cells in and of itself detrimental to neural survival or function?
Continue reading “￼Proliferative reactive gliosis is compatible with glial metabolic support and neuronal function”